How would you use a light microscope to look at onion cells?
1) To use a light microscope to look at onion cells, you should first prepare the slide: add a drop of water, the epidermal tissue of the onion with tweezers, and iodine solution for colour and clarity. You should then cover the slide with the cover slip carefully to prevent air bubbles, which can obstruct the view of the specimen.
2) You should then attach the slide to the stage.
3) You should then use the course adjustment knob to bring the lowest-powered objective lens to roughly above the slide.
4) If necessary, you should then turn on the light source.
5) You should then use the fine adjustment knob to increase the definition of the image.
6) If necessary, you should repeat this process with higher magnification objective lenses.
7) Finally, you should draw your observations, using a sharp pencil and using at least half the space available without colouring/shading. You should label the organelles with clear, uncrossed lines, and include a title and scale.