Balance between agressive amplification and avoidance of contaminating template and can be resolved physically and chemically?
Contaminants
What is the major cause of contamination?
PresenceofPCRproductsfromprevious amplifications
Methods of physical decontamination?
Separations
UV light
Method of physical decontamination that induces base damage that catalyzes the formation of single and double-stranded strands of DNA?
UVlight
UV light enhanced the effectivity with the addition of?
psoralens
Methods of chemical decontamination?
10% bleach
dUTP-UNG
Widely used method for decontamination and workspace preparation, 7mm hypochlorite solution, and it is frequently wiped on surfaces that come in direct contact with specimen material to remove most DNA contamination.
10% bleach
Method of chemical decontamination which substitutes the dTTP for dUTP in the PCR reagent master mix.
dUTP-UNG
Uracil-N-glycosylase (UNG) degrades any nucleic acid containing?
Uracil
The incubation period of the chemical decontamination method that is added before PCR amplification?