Lab Practical

• What is the medium that supports the growth of cells on a cell culture dish, and consists of vitamins, amino acids, glucose, and phenol red?

  Dulbecco's Modified Eagle Medium (DMEM)
• What is used to degrade extracellular matrix proteins in the process of collecting cells from a cell culture dish?

  Trypsin
• What types of shoes are not allowed to be worn in the lab?

  Open toed
• What is the saccharide binding protein found in Jack bean meal?

  Concanavalin A
• What is the glucose-based saccharide found in Sephadex?

  Dextran
• What is the enzyme that has an oligosaccharide core that contains mannose?

  HRP
• What solution was used as an eluent for the affinity chromatography column?

  NaCl/Dextrose
• What does Con A normally bind to?

  Mannose
• Proteins that do not bind to ligand in affinity chromatography are called?

  Flow through
• What type of ligand-interaction was observed within the affinity chromatography experiment?

  protein-ligand
• What was the matrix used to isolate the protein within the affinity chromatography experiment?

  Sephadex
• What is used to wash the DNA pellet within the mini prep DNA experiment?

  70% ethanol
• Which form of DNA runs the fastest on an agarose gel identified in the mini prep experiment?

  Supercoiled
• What type of buffer contains RNase to degrade RNA and resuspend precipitated DNA?

  Tris Buffer
• What voltage was the agarose gel run within the mini prep experiment?

  120 volts
• Which form of DNA runs the slowest on an agarose gel identified in the mini prep experiment?

  catenane
• What is the cation required for restriction enzyme activity?

  Magnesium
• What protocols utilize sticky and blunt end restriction enzymes?

  Molecular cloning
• What are palindromic sequences separated by several variable bases of DNA?

  hyphenated sites
• What was used in this experiment that causes nucleic acids to fluoresce upon exposure to ultraviolet light?

  Ethidium bromide
• What is the x axis labeled on the non logarithmic semi log graph paper used for RE mapping?

  Migration Distance
• What is the first step for mapping DNA restriction sites?

  determine migration distance and size of the DNA fragments
• What are antigens?

  macromolecules that elicit antibodies
• What does a monoclonal antibody recognize?

  Antigen
• What was conjugated to the secondary antibody within the western blot experiment?

  HRP
• What type of gel is run when performing a Western Blot?

  SDS-PAGE
• What protocol is used to determine the presence of certain antigens or antibodies?

  Western Blot
• What is a Western Blot used to determine?

  Protein antigens
• What was the protein that was analyzed in high and low concentration using a Western Blot in this experiment?

  BSA
• What are two ways during PCR you can get unwanted amplification products?

  sample contamination or nonspecific annealing of primers.
• How many total cycles were conducted in the pcr experiment?

  30
• What cation is required for Taq polymerase?

  Magnesium
• True or False: Each PCR cycle doubles the amount of target DNA in less than five minutes?

  True
• During electrophoresis, the DNA samples migrate towards what electrode?

  Positive
• If you start PCR with two copies of the template DNA, how many copies would you produce after 3 rounds?

  16 copies
• In order for this to be activated, both copies of the gene need to be mutated?

  tumor suppressors
• What specific oncogene is mutated in cancers seen within the lung, colon, and stomach?

  myc
• Most cancer cells result from what in genes controlling cellular growth?

  mutations
• This is triggered in a normal cell if severe DNA damage occurs within the cell cycle?

  apoptosis
• What is used to stain the nucleus of cells and bind to negatively charged molecules?

  Methylene Blue