General Principles

• Nucleic Acid Hybridization take advantage of the binding of single-stranded nucleic acids with their complementary sequences to identify specific DNA sequences.
• Hybrid can be?

  DNA-DNA

  DNA-RNA

  RNA-RNA
• Uses of Nucleic Acid Hybridization?

  Detection of specific sequences in a mixture of DNA fragments or gDNA

  Determination of base sequence similarities and changes
• 1. Target DNA
  2. Mixture of different DNA fragments
  3. Mix and allow to reanneal
  4. Probe DNA
  5. Usually homogeneous and labeled
  6. Reannealed target DNA
  7. Probe-target heteroduplexes
  8. Reannealed probe DNA
  9. Denature