HEMA - PRELIMS
Cards (9)
- 1657: Describe worms in the blood by Athanasius Kircher1658: Discovery of erythrocytes by Swammerdam1674: Human erythrocytes were described by Anton Van Leeuwenhoek1842: Guilio Bizzozero described platelets as “petites plaques”1846: PMN distinguished from other leukocytes by Wharton Jones1879: First complete classification of leukocytes by Paul Ehrlich1902: Development of Wright’s stain by James Homer Wright1920: Hematology was considered separate science from clinical pathology
- COMPOSITION OF BLOOD: Liquid portion: Serum or Plasma (55%) Cellular portion: Erythrocytes, Leukocytes, and Thrombocytes (45%)
- Average blood volume is 5 liters (adult)
- AUTOMATED ANALYZERS – differentiate and enumerate RBC’s
- ROMANOWSKY (WRIGHT’S) (polychromatic, a mixture of acidic and basic dyes)
- Uppermost layer: lightest, less dense (PLATELETS)
- Middlemost Layer: non-granulated WBC'S (LYMPHOCYTE AND MONOCYTE)
- Lowermost layer: granulated WBC'S (NEUTROPHIL, EOSINOPHIL, BASOPHIL, RETICULOCYTES, IMMATURE RBC, PRECURSOR CELLS)
- THROMBOCYTES/PLATELETS - 2-4um ERYTHROCYTES/RBC’S - 6-8um NORMAL LYMPHOCYTES - 6-9umREACTIVE LYMPHOCYTES - 10-22umBASOPHILS - 10-15umSEGMENTED NEUTROPHIL - 10-15umBAND NEUTROPHILS - 10-15umEOSINOPHILS - 12-16umMONOCYTES - 12-20um