Pyrosequencing

• Determines a DNA sequence wothout having to make a sequence ladder.

  Pyrosequencing
• Relies on the generation of light (luminescence) when nucleotides are added to a growing strand of DNA.

  Pyrosequencing
• Most useful for short to moderate analysis or Single Nucleotide Polymorphism (SNP) detection and typing rather than for generating new sequences.

  Pyrosequencing
• Reaction Mix:

  Single-stranded DNA template, Sequencing Primer, Sulfurylase, Luciferase, Luciferin, and Adenosine 5’ Phosphosulfate