Bisulfite DNA Sequencing

• Methylation-Specific Sequencing

  Bisulfite DNA Sequencing
• Important as methylation of DNA that affects cell differentiation and is implicated in a number of diseases such as cancer.

  Bisulfite DNA Sequencing
• Modification of chain termination sequencing to detect methylated nucleotides and is less concerned with generating a new sequence.

  Bisulfite DNA Sequencing
• gDNA is cut with restriction enzymes to facilitate denaturation and the fragments are resolved in?

  Agarose Gel Electrophoresis
• Fragments are denatured by heat and exposed to bisulfite solution (Sodium Bisulfite, NaOH, and Hydroquinone).
• Converted to uracil.

  Cytosines
• 5-methyl Cytosines


  Unchanged