Next Generation Sequencing

• Designed to sequence large numbers of templates simultaneously, yielding hundreds of thousands of sequences in jist a few hours.

  Next Generation Sequencing
• Goals:

  1. Make sequencing the entire genome accessible
  2. Make genomic studies a part of routine in research and the clinic
• How does illumina work?

  1. Fragments
  2. Acid Adaptors
  3. Attach to Flowcell
  4. Bind to Primer
  5. PCR Extension
  6. Dissociation
  7. Cluster Formation
  8. Sequencing
  9. Signal Scanning
• Types of Next Generation Sequencing

  Flongle

  MinION

  GridION

  PromethION
• Flongle : Maximum run time

  16 hours
• MinION : Maximum run time

  72 hours
• GridION : Maximum run time

  72 hours
• PromethION : Maximum run time

  64 hours
• Flongle : Theoretical 1D maximum yield

  Up to 3.3 Gb
• MinION : Theoretical 1D maximum yield

  Up to 40 Gb
• Grid ION : Theoretical 1D maximum yield

  up to 200 Gb
• PromethION : Theoretical 1D maximum yield

  Up to 15 Tb
• Flongle : Current 1D maximum yield

  Up to 2 Gb
• MinION : Current 1D maximum yield

  Up to 30 Gb
• GridION : Current 1D maximum yield

  Up to 159 Gb
• PromethION : Current 1D maximum yield

  Up to 8.6 Tb
• Flongle : Available channels

  Up to 126
• MinION : Available channels

  Up to 512
• GridION : Available channels

  Up to 2,560
• PromethION : Available channels

  Up to 144,000