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Biology
What is Microscopy?
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Created by
Emmanuella Osebre-Osei
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Microscopy
The use of
microscopes
How light microscopes work
1.
Light
from the room hits the
mirror
2.
Reflected upwards
through the object
3. Passes through the
objective
lens
4. Passes through the eyepiece lens
5. Into the
eye
Object
The
real object
or
sample
that you're looking at
Image
The image that we see when we look down the microscope
Magnification
How many times
larger
the
image
is than the object
Magnification =
image size
/
object size
Resolution
The shortest distance between
two
points on an object that can still be distinguished as
two
separate entities
Higher resolution
means more detail and less blurriness
Parts of a light microscope
Base
Arm
Light source
(lamp or mirror)
Stage
Objective
lenses
Eyepiece
lens
Body tube
Coarse
and
fine
focusing knobs