microscopy

• Microscope


  Device that uses light or electrons to magnify and view small objects
• Light microscope


  • Used to study cells
  • Allows us to make important discoveries about cell structures like the nucleus
• Light microscope limitations


  • Limited magnification
  • Limited resolution - blurred images even with high magnification
• Electron microscope


  Microscope that uses a beam of electrons instead of light to view specimens
• Electron microscope advantages


  • Much greater magnification than light microscope
  • Much higher resolution than light microscope
• Calculating microscope magnification


  Magnification = Size of image / Size of real object
• Calculating magnification


  • Nucleus image size = 45 mm, Actual nucleus diameter = 0.01 mm, Magnification = 4500x
• Calculating magnification


  • Mitochondrion image size = 30 mm, Actual mitochondrion length = 0.003 mm, Magnification = 10000x
• Calculating real object size

  • Image size = 87 mm, Magnification = 2000x, Real cell size = 0.0435 mm
• Microscopes allow us to magnify and view small objects
• Light microscopes have limitations in magnification and resolution
• Electron microscopes have much greater magnification and resolution than light microscopes
• Magnification = Size of image / Size of real object