Hematology 2

• Complete blood count

  • set of medical laboratory tests
  • counts of WBC RBC Platelets
  • concentration of hemoglobin and hematocrit
• RBC Pipette

  • 0.5 - 1 - 101
  • Large bulb
  • Red bead
  • Red mouth piece
• WBC Pipette

  • 0.5 - 1 - 11
  • Small bulb
  • white bead
  • white mouth piece
• Bulb

  • for mixing and diluting fluid
• Bead

  • helps in mixing blood and diluting fluid
  • indicates the bulb is dry or wet
  • helps to identify RBC or WBC Pipette
• RBC counting

  • measures the number of RBC
  • suck blood until 0.5
  • get medium until it reaches 101
  • Observe under HPO
• L rule

  • Left and bottom only
• RBC count = Sum of number of cells in 5 squares x 10,000
• Normal RBC count

  Men: 4.7 - 6.1 Million

  Women: 4.2 - 5.4 Million
• Low RBC Count Erythropenia

  • Anemia
  • Vitamin deficiency
  • internal bleeding
  • kidney disease
  • malnutrition
• High RBC Count Polycythemia

  • smoking
  • heart disease
  • dehydration
  • hypoxia
  • lung disease
• WBC Counting

  • measures number of WBC
  • Observed in LPO
  • grower‘s medium
• WBC Count = sum of number of cells in 4 squares x 50
• Normal total WBC count

  Adults: 4000 to 11000
• Low Total WBC count Leukopenia

  • liver or spleen disease
  • Radio and Chemotherapy
  • Severe bacterial Infection
  • autoimmune disease
  • failure of bone marrow
  • tuberculosis
• High total WBC count Leukocytosis

  • Smoking
  • stress
  • bacterial infection
  • Leukemia
  • tissue damage
  • drugs and medication
  • inflammatory disease
  • spleen removal
• Hematocrit

  • percentage by volume of RBC
• Normal Blood

  Women : 37-47%

  Men: 42-52%
• Materials used for hematocrit

  • sealant
  • ethanol
  • ruler
  • centrifuge
  • capillary tube
• Hematocrit = Measurement of RBC / overall measurement x 100