Glucose

• CLINICAL LABORATORY FINDINGS
• The amount of colored complex is proportional
• Postprandial


  After eating
• Normally no detectable glucose in urine
• Interval corrected for albumin: 191-265 μmol/L

  
• Enzymatic assay: 151-300 μmol/L
• Condensation method


  • Ortho-toluidine (Dubowski)
• Blank


  Add distilled water
• Standard


  Add standard
• Control


  Add Level 1 control
• Test


  Add serum
• Chemical method principle is dehydration and oxidation
• Phospholipid Measurement


  Chemical Method
• Characterized by: neurologic symptoms, mental retardation, self-mutilation
• Familial juvenile hyperuricemic nephropathy
• G6P deficiency (glycogen storage disease)
• F1Paldose deficiency (fructose intolerance)
• Purine nucleoside phosphorylase deficiency
• Association with Alzheimer's and Parkinson's
• Negative interferences


  • Hemoglobin and xanthine
• Fasting specimen is not required
• Interferences occur in the presence of lipemia
• Calculations


  Conc. of test (g/dL) = Absorbance of test / Absorbance of standard x Conc. of standard
• Sample


  1. 5 μL
• Run time


  40-60 mins
• Parameters for HPE


  Agarose gel
• Sample


  1. 5 μL
• Parameters


  Agarose gel
• IgG light chains


  Jones protein
• green


  pyrogallol red
• Instrument


  Handheld refractometer
• Electrophoresis


  When abnormality is found