1. Set up a source of light at a specific distance from the plant
2. Measure the volume of gas produced by the plant over a set time
3. Analyze the proportion of oxygen in the gas produced
Not enough light slows down the rate of photosynthesis
As light intensity increases
The rate of photosynthesis increases steadily up to a point
Beyond a certain light intensity, further increases won't affect the rate of photosynthesis
If light is not the limiting factor, it will be either temperature or CO2 level
In the lab, light intensity can be changed by moving a lamp closer to or further from the plant
To get a graph showing the relationship between light intensity and rate of photosynthesis, you need to measure light intensity using a light meter or do calculations
Too little CO2 also slows down the rate of photosynthesis
As CO2 concentration increases
The rate of photosynthesis increases up to a point
After a certain CO2 concentration, the rate of photosynthesis no longer increases
If light and CO2 are in plentiful supply, then temperature is the limiting factor
Usually, if temperature is the limiting factor, it's because it's too low
If the plant gets too hot, the enzymes needed for photosynthesis and other reactions will be destroyed, usually around 45°C
A graph could show the effect of both light intensity and temperature on the rate of photosynthesis
The line for the effect of light intensity would level off at a higher point than the line for temperature, indicating that temperature is the limiting factor
A graph could also show the effect of both light intensity and CO2 concentration on the rate of photosynthesis
The line for the effect of CO2 concentration would level off at a higher point than the line for light intensity, indicating that CO2 is the limiting factor
The Canadian pondweed experiment can be used to measure the rate of photosynthesis
Canadian pondweed experiment
1. Set up a source of light at a specific distance from the plant
2. Measure the volume of gas produced by the plant over a set time
3. Analyze the proportion of oxygen in the gas produced
The apparatus used in the Canadian pondweed experiment can be altered to measure the effect of factors like CO2 on the rate of photosynthesis
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