Required practical 5: Investigate the effect of pH on the rate of reaction of amylase enzyme
1. Add 10 cm³ of a 4% starch solution to a boiling tube
2. Add 10 drops of iodine solution and stir using a glass rod
3. In a separate test tube, add 2 cm³ of a 2% amylase solution
4. To the test tube containing the amylase, add 5 cm³ of the first buffer solution to be tested
5. Leave both tubes in a water bath at 35 °C for 2 minutes
6. Add the amylase and buffer solution from the test tube to the starch solution in the boiling tube, and stir
7. Remove one drop of the solution every 30 seconds and place it into a well on the spotting tile
8. Repeat the experiment using the different pH buffers provided
