RP:Microscopy (B.1) (B.41)

• What is aim of this investigation?

  To investigate and view the sub-cellular structures of plant and animal cells using a microscope.
• What type of microscope is used to view the cells?

  Light microscope
• what type of tissue issued?

  A thin layer of onion skin
• how is the slide prepared?

  1)drop of water added to glass slide 2)thin layer of onion skin placed onto glass slide 3)drop iodine added onto the onion skin 4)cover slip placed on top
• what do we need to ensure is not present on the slide?

  Air bubbles
• why is iodine used to prepare the slides?

  To dye the sub-cellular structures and make them easier to see
• which magnification is used to first view the cells?

  x10
• how do you first find the cells under the microscope?

  By turning the coarse-focusing wheel
• which sub-cellular structures should you be able to identify?

  Nucleus, cell wall, vacuole, cell membrane and cytoplasm
• which structures cannot be seen with a light microscope?

  ribosomes, mitochondria
• what type of microscope would you need to use to improve the resolution of the image?

  Electron microscope
• formula to calculate the magnification?

  Magnification = size of image / size of real object
• how do you rearrange the formula to find the size of the real object

  size pf the real object = size of image / magnification
• how do you convert from mm to um?

  Multiply by 1000.