Investigating the effect of pH on the rate of enzyme activity
1. Put a drop of iodine solution into every well of a spotting file
2. Place a Bunsen burner on a heat-proof mat, and a tripod and gauze over the Bunsen burner. Put a beaker of water on top of the tripod and heat the water until it is 35 °C
3. Use a syringe to add 1 cm³ of amylase solution and 1 cm³ of a buffer solution with a pH of 5 to a boiling tube
4. Use a different syringe to add 5 cm³ of a starch solution to the boiling tube
5. Immediately mix the contents of the boiling tube and start a stop clock
6. Use continuous sampling to record how long it takes for the amylase to break down all of the starch
7. Repeat the whole experiment with buffer solutions of different pH values to see how pH affects the time taken for the starch to be broken down
8. Control any variables each time (e.g. concentration and volume of amylase solution) to make it a fair test
