Hema

• blood mixing - for all hematologic procedures.
• Packed cell volume - using microhematocrit to obtain HCT.
• plasma protein estimation - refractomy. for vet only
• Principle of CBC automated analyzer - Electrical impedance and flow cytometry
• primary square - 9

  secondary - 16

  tertiary - 25
• cephalic vein - dextrose
• jugular vein - most common site for MAMMALS
• EDTA - hematological studies
• red top - chemistry
• green top - blood gas analysis
• blue top - coag studies
• Balance heparin syringes with cups - for electrochemical test collection
• diameter of human rbc - 8-9um
• normal mcv of human - 80-100 ml
• Rbc of mammals - Anucleated erythrocyte
• Cammelidae - oval erthrocyte.
• Canine - similar characteristics and lifespan to humans
• mild polychromasia - Canine
• Life span of Canine rbc - 110-120 days
• Feline - little to no central pallor.
• life span of feline rbc - 65-76 days
• Anisocytosis - Rbc variation in size
• macrocytosis and microcytosis

  
• variation in shape - Poikilocytosis
• Color variation - Chromosia
• hypochromic and polychromic
• Hypochromic - IDA, chronic blood loss and anemia
• Polychromic - anemia, bone marrow disease
• Difff quick stain - purpulish
• New methylene blue - bluish
• Also known as the spurr cells - ACANTHOCYTES
• Crenated cells - ECHINOCYTES
• blunt or club-shaped projections - Acanthocytes
• Hemangiosarcoma - ACANTHOCYTES
• seen in horses - echinocytes
• Helmet cells or bite cells - KERATOCYTES
• seen in cats - keratocyte
• horn like - SCHISTOCYTES
• Mouth like - STOMATOCYTE
• Mexican hat cell or target cell - CODOCYTES