effect of antibiotics

Cards (2)

  • Required Practical 2 – Investigating the Effect of Antibiotics
    1. Spray bench with disinfectant
    2. Mark agar plate
    3. Wash hands
    4. Put antiseptics on filter paper discs
    5. Place discs on agar plate
    6. Note which antiseptic is in each section
    7. Secure lid with tape
    8. Incubate plate at 25°C for 48 hours
    9. Measure diameter of clear zones around discs
    10. Record results in a table
  • Do not seal the lid all the way around as this creates anaerobic conditions. Anaerobic conditions will prevent the E. coli bacteria from growing and can encourage some other very nasty bacteria to grow.