MC3 LAB

• Ascaris lumbricoides

  
• Trichinella spiralis

  
• Wuchireria bancrofti

  
• Enterobius vermicularis

  
• Trichiuris trichiura

  
• Chemical compounds

  • Nitrogen
  • Carbon
  • Energy
  • Minerals
  • Growth factors
  • Buffer
  • Indicator dye
  • Reducing substance
  • Selective agents
• Nutrient broth

  • Sugar
  • Amino acids
  • Vitamins
  • Other growth factors
• Solid medium


  • Agar
  • Agar-agar shreds
• Materials


  • Beet extract
  • pephine
  • -agar
  • weighing scale
  • sterilizer
• Beet extract


  • 0.3
  • 0.52
• Beet extract composition

  • amino acids
  • vitumine
  • mins
• Reptone


  Partially digested protein-source
• Reptone is a source of nitrogen and carbon
• Preparing growth medium


  1. 1L Water 3g BE; Sg P&P NAB

  2. 1L NAB 15-20g A = NA
• Agar


  • Gel-forming polysaccharide from red seaweeds (aggrophytes)
  • Mix of agarose and agato pectine
  • Molten and fluid at 40-45°c
• Agar gel-forming polysaccharide
• Mrs Jenifer Ramallosa /Mr. Joe Merfurt Lama