Structure and function of Albumin

• What are the major function of human serum albumin?

  Transporter of smaller, mostly hydrophobic molecules

  Major contribuutor (80%) of osmotiv pressure of blood pressure
• which electrode does albumin migrate to?

  The positive electrode
• what is a major reason other molecules are found with albumin?


  the are associated with it
• what type of data is used with forest plots?

  Meta-analysis
• what does odds ratio mean when =1?

  no effect
• what does odd ratio mean when <1?

  control better than test
• what does odds ratio >1?

  test better than control
• What is hypoalbuminemia mean for a patient?

  increase incidence of disease both non-communicable and communicable'
• what are the structure types of human serum albumin?

  alpha helices

  B-sheets

  quaternary structure

  disulphide bonds

  metilation
• Is an alpha helix common in human serum albumin?

  yes but is only marginally stable in solution
• How are alpha helices stabilised in human serum albumin?

  packed pairwise in proteins with their hydrophobic residues inwards
• how many polypeptide chains make up human serum albumin?

  1
• how many intra-chain disulfide bonds in serum albumin?

  17
• how many similar domains are there?

  3
• what are the types of loops found in human serum albumin?

  S loops and L loops
• What is the albumin structure?

  LSL loop (long, short, long loops)
• what is cystine?

  two cysteine joined together by a disulfide bond
• which amino acid is in a unusally high percentage in albumin?

  Cysteine
• what are the binding sites for albumin for?

  small heterocyclic or aromatic carboxylic acids and metals
• what does cys-34 bind to?


  Cd, Au, Hg, Ag (ions)

  The N-terminal His-3 binds tp Cu(II), Ni(II)
• wich part of cys-34 binds to ions?

  SH-groups
• what are transported in albumin?

  Hormones and drugs (aspirin, AZT, penicillin and warfarin)
• what damages other chemicals in the blood?

  oxygen
• what is the shape of human serum albumin?

  Heart shape
• how many free fatty acids can bind to albumin?

  5
• what occurs when a fatty binds?

  there a conformation change
• how many alpha helices hold in hydrophobic fatty acids?

  3 or 4
• what makes us feel pain?

  arachidonic acid - regulated by aspirin
• which chain binds metals?

  main chain and side chain
• what does propofol do?

  has an anaesthetic
• where does ibuprofen bind to?

  serum albumin (2bxg)
• what are a good nucleophile?

  Free thiols
• what can albumin be used for?


  imaging
• what is evans blue?

  dye that binds to albumin
• what are the excitation peaks of evans blue?

  470 and 540nm
• what is emission peak of evans blue?

  680nm
• when does albumin reach tissue?

  when blood vessels are broken
• what are the binding site of HSA?

  Fatty acids, drugs, and metal ions.

  A) thyroxine and propfol

  B) hemin, bilirubin, fusidic acid and lidocaine

  C) NO, Au+, Pt2+

  D) throxine, indoxul sulpfate, CMPF, warfarin