evidence for fluid mosaic?

Cards (3)

  • first model? 3 layer protein sandwich.
    bad as didn't allow heads to be in contact with water or amino acids on outside to be kept away from water.
    • research into lipid bilayer formation used to refine model
  • freeze fracture?
    smooth mosaic like surface seen and dispersed is molecules like proteins/lipids.
    • used molecule lectin labelled with ferritin which is visible under electron microscope, only binds to polysaccharides, lectin only bound to outside of membrane so outside is different from inside
  • fusing of human/mouse cells?
    mouse cell labelled w/ green pigment for protein, humans labelled w red pigment for protein.
    • 2 cells allowed to fuse, immediately after fusing proteins stayed in respective places but after 40 mins 37 degrees. complete mix of protein.
    • could've only mixed via diffusion through surface so membranes are fluid.