Techniques

• Ziehl-Nielsen technique was formerly used for acid fast stains where it involved heating the Carbolfuschin.
• Kinyoun method is now used for acid fast stains in which it incorporates a detergent tergitol
• Broth to Broth transfer: Flame loop, Remove lids and flame the lip of tube and make transfer. Lastly, reflame lip of tubes and flame out.
• Streak plates are used to isolate colonizes