Preparing and observing slides
1. Add a drop of water to a clean slide
2. Carefully cut an onion and separate it into layers
3. Use tweezers to peel off a piece of epidermal tissue and place it on the slide
4. Add a drop of iodine solution as a stain
5. Place a cover slip over the specimen carefully to avoid air bubbles
6. Secure the slide on the stage of the microscope
7. Begin with the lowest-powered objective lens
8. Adjust the coarse adjustment knob to bring the stage close to the lens without touching
9. Look through the eyepiece and use the coarse adjustment knob to find the focus
10. Fine-tune the focus with the fine adjustment knob for a clear image
11. Switch to a higher-powered objective lens and refocus for greater detail