MICROSCOPY

• Microscopy


  The field of using microscopes to view objects and even smaller parts of objects that cannot be seen with the unaided eye

  
• Microscope

  An optical instrument that produces larger images or pictures of specimens

  
• Hans & Zacharias Janssen


  • Dutch eyeglass makers, inventors of the first compound microscope (2 lenses), tube with lenses at each end in 1590

  
• Antonie van Leeuwenhoek


  • Invented the simple microscope

  
• Robert Hooke

  • English chemist, mathematician, physicist, and inventor who contributed to the improvement of the compound microscope

  
• Compound light microscope

  Can magnify tiny objects up to about 1000 times their actual size
• Parts of a compound light microscope

  • Base
  • Arm
  • Body tube
  • Stage
  • Eyepiece
  • Eyepiece tube
  • Diaphragm
  • Nosepiece
  • Objectives
  • Adjustment knobs
  • Mirror

  
• Base

  Supports the microscope
• Arm

  The main frame to which the base and all the other parts are attached
• Body tube

  Connects the eyepiece to the objective lenses and maintains the correct distance between the eyepiece and the lenses
• Stage

  Supports the microscope slide and the stage clip
• Eyepiece

  Where you look through to view the object
• Eyepiece tube

  Holds the eyepiece lens in place
• Diaphragm

  A wheel-shaped device that regulates the amount of light passing through the specimen
• Nosepiece

  A revolving part that carries the objective lenses
• Objective lenses

  • Oil immersion objective (100x)
  • Low-power objective (10x)
  • High-power objective (40x)
  • Scanning objective lens (4x)
• Adjustment knobs

  The coarse adjustment knob for larger focusing and the fine adjustment knob for precision focusing
• Mirror


  Reflects light to the lens of the microscope
• HPO is blue

  OIP is white

  LPO is yellow

  SOL is red
• stage clips support the microscope slide
• how many lenses are there in Leeuwenhoek's microscope?


  1 lens