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• Blood Donation

  A process of collection, testing, preparation, and storage of blood from donors who are usually volunteers
• Types of Blood Donation

  • Direct Blood Donor
  • Autologous Donation (donation is for the benefit of the donor himself / herself who will use the same during his / her scheduled surgery)
• Blood-related Definitions

  • Blood/blood product
  • Blood bank/center
  • Commercial blood bank
  • Hospital-based blood bank
  • Blood collection unit
  • Voluntary blood donor
  • Walking Blood Donor
  • Blood transfusion transmissible diseases
• Goals of Blood Donor Phlebotomy

  • Ensure the safety of the donors
  • Minimize and prevent contamination in the donated blood which may come from external sources
  • Conduct safe collection of donated blood for therapeutic purposes especially during its shelf life
  • Make sure that other personnel are well-trained and qualified to do the venipuncture procedure for blood donation
• Requirements for Blood Donation

  • At least 50 kg
  • 16 - 65 years old
  • 16 - 17 years old must secure a written consent from a parent or legal guardian
  • 61 - 65 years old must be a frequent donor
• What to do before donating blood

  • Sleep for at least 7 hours
  • Avoid smoking 4 hours before blood donation
  • Do not take any alcoholic drinks a day before donation
  • Avoid fatty food
  • Eat a light and healthy meal 3 - 4 hours before donation
  • Drink plenty of water
  • Bring any Valid ID and COVID-19 vaccination card
• Blood Donation Procedure

  1. Identify the donor & label the collection bag and test tubes

  2. Select the vein

  3. Perform Hand Hygiene and Wear Well-Fitting Gloves

  4. Disinfect the Donor's Skin

  5. Perform the Venipuncture

  6. Monitor the Donor and the Donated Unit

  7. Remove the Needle and Collect the Laboratory Samples
• Component of 1 Unit of Blood

  • 405 to 495 mL of Blood
  • 63 mL of Anticoagulant and preservative – CPD or CPDA
  • 3 main blood components: Packed Red Blood cells (PRBC), Plasma, Platelets
• Responsibilities of the Phlebotomist

  • Donor interview and screening
  • Donor identification
  • Donor informed consent
  • Venipuncture
  • Product collection
  • Post-donation care
  • Complication management
• Donor Selection Tests


  • ABO Blood group
  • Rh blood type
  • Bloodborne pathogens: Chagas disease, HIV 1 and 2, Hepatitis B, Hepatitis C, Syphilis, West Nile virus, Human T-cell lymphotropic virus, Malaria
• Donor Registration and Identification Information

  • Name
  • Address
  • Contact number
  • Date of birth
  • Social security number (if applicable)
  • Sex
  • Date of last donation (at least 8 weeks or 120 days is required between whole blood donations)
• Eligibility Requirements for Blood Donors

  • Appear to be in good health
  • No evidence of skin infections, rashes, or track marks
  • 17 years old, or in some states 16 years old with parental consent
  • Weigh at least 110 pounds
  • Temperature of 99.5°F or lower
  • Blood pressure no higher than 180/100 mm Hg
  • Pulse rate between 50 and 100
  • Hemoglobin level of 12.5 g/dL or higher or a hematocrit of 38% or higher
• Medical History Interview Questions

  • Past and current medications and antibiotics
  • Injected drug use, exposure to infectious diseases
  • History of infectious disease, venereal disease
  • Social habits, particularly related to bloodborne pathogen exposure
• diseases which may be transmitted as a result of transfusion

  Blood Transfusion transmissible Diseases