Decay Required Practical

Cards (1)

    • Required practical - Decay:
    1. Label 5 test tubes as 'lipase' and add 1cm33 of lipase to each
    2. Take another 5 test tubes and add 5 drops of phenolphthalein
    3. Add 5cm33of full fat milk to the indicator test tubes and label them 'milk'
    4. Using a pipette, add 7cm33 of sodium carbonate solution to the milk (solution should turn pink)
    5. Place a test tube of lipase and milk into the water baths until they are of the desired temperatures
    6. Start with the lipase and milk at 20ooC
    7. Transfer the lipase to the milk test tube. Start the stopwatch immediately and begin gently stirring
    8. Continue stirring the contents until the pink colour disappears
    9. Record the time taken for the pink to disappear in the results table
    10. Repeat stages 2 - 4 for the other temperatures
    11. Put info in a table and calculate a mean for each temperature