L2 - Microscopy

• Scale

  A) centi

  B) milli

  C) micro

  D) nano

  
• 1 order of magnitude is 10x, this means that 3 orders of magnitude is 1000x
• The sample you are looking at on a microscope is called the object.

  When looking through the microscope, you will see the image.
• Magnification is how many times larger the image is shown than the actual object

  Resolution is the shortest distance between two objects that can be seen clearly
• Types of microscopes - Light microscopes and Electron microscopes, the image below shows a light microscope, label it:

  A) objective lens

  B) stage

  C) eyepiece lens

  D) focusing wheel

  E) light source

  
• Pros of light microscopes:

  • Easy to use
  • Relatively cheap
• Cons of light microscopes:

  • Relies on light
  • Resolution is limited
  • Magnification is limited
• Pros of electron microscopes:

  • Uses electrons instead of light
  • Resolution is much higher
  • Magnification is much higher
• Cons of electron microscopes:

  • Very expensive
  • Hard to use (mostly used in labs)
  • No colour
• Equation of magnification:

  $magnification =$$image size / object size$
• Question: If the image size is 3cm and the object size is 0.05mm, what is the magnification?

  Answer: 600x
• Question: If the magnification is 3000x and the object size is 80nm, what is the image size in mm?

  Answer: 0.24mm