Monoclonal antibodies

• Monoclonal antibody

  Antibodies produced from a single clone of hybridoma cells
• Production of monoclonal antibodies

  1. Inject mouse with antigen

  2. Collect lymphocytes from mouse

  3. Fuse lymphocyte with tumor cell to form hybridoma

  4. Select single hybridoma cell producing desired antibody

  5. Allow hybridoma cell to divide by mitosis to form clone
• Monoclonal antibodies

  • Specific to one binding site on one protein antigen
  • Can target specific chemicals or cells in the body
• Monoclonal antibodies have a large number of uses
• There are plenty of questions on monoclonal antibodies in the revision workbook