Investigating the effects of temperature on decay by measuring the pH change of fresh milk in the presence of the enzyme lipase
1. Make a solution of milk and phenolphthalein indicator
2. Add sodium carbonate to make the solution alkaline and pink
3. Place the tube in a water bath at a specific temperature
4. Add the lipase enzyme and start the stopwatch
5. Time how long it takes for the pink to disappear (pH decrease)
6. Repeat at different temperatures