Bacteriology & Sensitivity

• Agar plates should be stored in the fridge (-5oC) before use
• Nutrient agar is suitable for growing E.coli
• Salmonella can be cultured on selentine broth
• Sabouraud's agar can be used to grow fungi
• Sabouruads agar changes from yellow to red in the presence of a pathogne
• Quadrant streak method can be used to inoculate an agar plate
• Methylene blue belongs to a simple group of stains
• Gram negative bacteria stains pink/red
• Gram positive bacteria stains purple/blue
• Ziehl-Neelson stain can be used to identify Mycobacterium spp.
• Using Ziehl-Neelson staining, acid fast bacteria stains red
• During antimicrobial sensitivity testing an agar plate should be incubated for 18-24 hours