Insertion and transformation - in vivo

• What does in vivo mean?


  In living organism
• What happens if the same restriction enzyme is used to cut DNA?

  All fragments produced will have ends complementary to one another so they can be stuck together
• What enzyme binds the sticky ends together?

  DNA ligase
• What is the promoter region?


  The binding site for RNA polymerase in transcription
• What transports the DNA into the host cell?

  Vector
• What is the most common vector?

  Plasmid
• How is the plasmid with the new DNA put back in the bacterial cell?

  The plasmids and bacterial cells are mixed together in a medium containing calcium ions

  This makes the membrane permeable so plasmids can pass in