The complete removal of microorganisms and their spores from the surface of an object
Pathogens
Disease-causing microorganisms
Any medical procedure that involves penetration of body tissues (an invasive procedure) requires the use of surgical aseptic technique
Below the waistline is considered unsterile
Tracheostomy involves the removal of the trachea
Linen refers to the patient's soiled clothing
IVP stands for Intravenous Pyelography
Medical asepsis
Any practice that helps reduce the number and spread of microorganisms
Zones in the surgical environment
Zone 1 (unrestricted)
Zone 2 (semi-restricted)
Zone 3 (restricted)
Radiographers in the surgical environment
Must follow protocols designed by OSHA
Shoes must be closed-toe and not cloth covered
Personal hygiene must be meticulous
Jewelry, long/artificial nails, and nail polish are prohibited
Body piercing jewelry must be removed
Hair, beards, and mustaches must be covered
Shoes must be protected from contact with body fluids
Must scrub hands and arms before entering Zone 3
Must don a mask before entering a room where a surgical procedure is in progress
Members of the surgical team
Surgeon
Surgical Assistant
Anesthesiologist
Nurse Anesthetist
Circulating Nurse
Scrub Nurse/Technician
Radiologic Technologist
Disinfection
The elimination of as many microorganisms as possible
Sterilization
The process of killing all microorganisms and their spores
Methods of sterilization
Chemical
Dry heat
Gas
Plasma technology
Autoclaving (steam)
Chemical sterilization
Involves immersion and soaking of clean objects in a germicidal solution followed by a sterile water rinse
Chemical sterilization is one of the less satisfactory methods for providing surgical asepsis and is not recommended
Dry heat sterilization
Required to sterilize some sharp instruments, takes 1-6 hours at 329°F-338°F (165°C-170°C)
Boiling water at 100°C has a boiling point of 78°C
Conventional gas sterilization
Uses Freon and ethylene oxide at 135°F (57°C), used for items like telephones and stethoscopes
Conventional gas sterilization has the disadvantage of producing toxic fumes
Gas plasma technology
A new and safer method of sterilization that uses vaporized hydrogen peroxide subjected to radiofrequency energy, used for endoscopes, fiberoptic devices, and microsurgical instruments
Gas plasma technology has the advantage of not producing toxic fumes
Autoclaving
Steam sterilization under pressure, the quickest and most convenient means of sterilization, at 250-275°F (121-135°C)
Sterile field
A microorganism-free area prepared for the use of sterile supplies and equipment
Criteria for a sterile package
Clean, dry, and unopened
Expiration date not exceeded
Sterility indicators have changed to a predetermined color
Skin preparation
The purpose is to minimize the introduction of pathogens to the body via a puncture or incision, thus reducing the likelihood of infection
Solutions used for skin preparation
Betadine
Zephiran
Standard principles of surgical asepsis
Any sterile object or field touched by an unsterile object or person becomes contaminated
Never reach into a sterile field
Suspect an item is contaminated, discard it
Do not pass between the physician and the sterile field
Never leave a sterile area unattended
1-inch border at the perimeter of the sterile field is considered a "buffer zone" and treated as contaminated
Removing and applying dressings
1. Perform hand hygiene
2. Don gloves
3. Inform the patient
4. Practice care in removing the dressing
5. Place soiled dressing in a plastic bag and seal before adding to biohazard container
Microorganisms that live on or inside the body without causing disease
Microbial flora
Types of microorganisms
Bacteria
Viruses
Protozoa
Prions
Fungi
Helminths
Most microorganisms do not cause infection or disease and are essential for our well-being
Pathogens
Microorganisms that cause infections and disease
Bacteria
Colorless, minute, one-celled organisms with a typical nucleus, classified as gram-positive or gram-negative
Gram-positive bacteria
Non-acid fast, retain dye when treated with alcohol, e.g. streptococci, staphylococci
Gram-negative bacteria
Acid-fast, dye is washed out by alcohol, e.g. E. coli, Bacillus, Mycobacterium tuberculosis
Endospores
The ability of bacteria to generate a resistant form to survive unfavourable environments
Significant diseases caused by bacteria
Tuberculosis
Streptococcal pharyngitis and necrotizing fasciitis