Biol 115

• Describe the principle of size exclusion chromatography

  It has resin made from insoluble polysaccharides which form a mesh.

  Only small molecules can pass through the mesh and the large molecules are excluded via the bead matrix.

  This means that proteins which an Mr higher than the molecular weight cut off elute quickly.

  The smaller molecules travel through the resin and so travel at a smaller rate causing them to elute last.