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Btec science unit 3
Test for protein
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Cards (6)
equipment
A test tube
A 10cm° measuring cylinder
Biuret solution
Step 1
Add a few drops of Bluret's reagent
sodium hydroxide
and
copper
(i) sulphate) to the food
sample in a
test tube.
Step 2
Shake the
solution
to
mix
and wait for a few minutes.
Step 3
If
protein
is present, the solution turns from blue to
purple.
risks
copper sulphate is
poisonous
and sodium hydroxide (corrosive).
. Wash immediately if comes into contact with skin and wipe away any spills to surfaces.