Microscopy

• Light microscope


  • Used to study cells
  • Allowed important discoveries about cell structures like the nucleus
• Light microscope limitations


  • Limited magnification
  • Limited resolution - blurred images even with increased magnification
• Electron microscope


  Has much greater magnification and resolution than light microscope
• Image taken with electron microscope


  • Showing detail in the nucleus that could not be seen with light microscope
• Calculating magnification


  Magnification = size of image / size of real object
• Calculating real object size

  Real object size = size of image / magnification