Microscopy

• Light microscope


  • Used to study cells
  • Allowed important discoveries about cell structures like the nucleus
• Light microscope limitations


  • Limited magnification
  • Limited resolution - blurred images even with increased magnification
• Electron microscope


  Has much greater magnification and resolution than light microscope
• Image taken with electron microscope


  • Showing detail in the nucleus that could not be seen with light microscope
• Calculating magnification


  Magnification = size of image / size of real object
• Calculating magnification


  • Image size = 45 mm, real object size = 0.01 mm, magnification = 4500x
• Calculating magnification


  • Image size = 30 mm, real object size = 0.003 mm, magnification = 10000x
• Calculating real object size

  Real object size = size of image / magnification
• Calculating real object size


  • Image size = 87 mm, magnification = 2000x, real object size = 0.0435 mm