BUN

• Lipase-Glycerokinase coupled with


  1. Glycerophosphate-oxidase

  2. Peroxidase
• Fasting samples are not required
• Urea-producing bacteria can break down urea
• Citrate, NaF


  Must be avoided
• Urease-producing bacteria can break down urea
• Prolonged standing causing clouding indicates bacterial multiplication
• Normal values: Healthy ambulatory adults: 7-18 mg/dL
• Adults >60: Plasma/serum 8-23 mg/dL, 2.9-8.2 mmol/L
• BUN-creatinine reference range: 10:1 to 20:1