Enzyme activity is affected by pH and temperature. If both factors increase over the enzyme's optimum condition, the enzyme activity will decrease and denature.
Enzymes are made up of biological catalysts - substances that increase the rate of chemical reactions without being used up. The shape of the active site is specific for each substrate (substance the enzyme acts on)
The active site has an optimal shape to fit with its corresponding substrate
When the substrate fits into the active site it forms an enzyme - substrate complex which then breaks down into products