Molecular typing

• Why should we use molecular typing systems?

  • Greater discrimination- organisms have many molecules, the more molecules analyses, the greater the discrimination
  • Differentiates between lots of molecules- nucleic acids, proteins, lipopolysaccharides
• Molecular typing is typically used to differentiate between members of the same species
• Molecular typing is split into two categories: pattern based typing and sequence based typing
• Pattern based typing includes:

  1. Plasmid profiling
  2. Ribotyping
  3. Pulse-field gel electrophoresis
  4. RAPD
• Sequence based typing includes:

  1. Multi Locus Sequence typing
  2. Whole genome sequencing