REQUIRED PRACTICALS

• MICROSCOPY - Put specimen on a microscope slide

  to view onion cells:

  add 1 drop of water to clean slide

  cut up onion and seperate into layers,use tweezers to peel of some epidermal tissue from the bottom of one of the layers

  Place epidermal tissue into water on slide

  add 1 drop of iodine solution (is a stain)

  place a cover slip on top stand cover slip upright on the slide and carefully tilt and lower so it covers the specimen with no air bubbles
• MICROSCOPY 2 - Clip prepared slide into the stage

  select lowest powered objective lens (least magnification)

  use the coarse adjustment knob to move stage up to judt below objective lens

  look down the eyepiece,adjust until image is roughly in focus

  adjust with fine adjustment knob until a clear image
• MICROSCOPY 3 - draw what you see under the microscope using a pencil

  do not include colour or shading

  subcellular structures should be in proportion

  include a title

  label important features
• Effect of pH on Enzyme Activity -

  1 drop of iodine solution into every well of a spotting tile

  place a bunsen burner on a heat proof mat,and a tripod and gauze over the burner. put beaker of water on top of tripod and heat the water until its 35*C

  add 1cm(cubed) of amylase solution and 1cm(cubed) of a buffer solution with a pH of 5 to a boiling tube. using test tube holders,put the tube into beaker of water for 5 mins