Microbiology

• Step 1: sterilise inoculating loop using a bunsen burner's roaring flame
• Step 2: Dip disks into antiseptic solutions leaving one out
• Step 3: Put inoculating loop into bacteria gel
• Step 4: under the agar plate divide it into the same parts as the disk number with a marker
• Step 5: open lid slightly next to a bunsen burner (to create a sterile field)
• Step 6: slowly use the inoculating loop across the plate
• Step 7: put a disk on one of the divisions
• Step 8: Close lid and tape it leaving a part tape less (prevents anaerobic respiration)
• Step 9: store at 25°C for 48 hours upside down (prevent condensation from falling on plate)
• Step 10: Measure zone of inhibition of each disk
• Step 11: compare zones of inhibition of each disk