Cell Fractionation

• Why is cell fractionation used?

  To study a specific cell organelle in a tissue sample
• Stages of cell fractionation

  1. Homogenisation
  2. Filtration
  3. Ultracentrifugation
• Purpose: Homogenisation

  Breaks up the cells in a tissue sample
• Purpose: Filtration

  Removes large cell debris and unbroken cells
• Purpose: Ultracentrifugation

  Separates cell organelles by mass