DNA, RNA, PROTEINS

• In a dividing eukaryotic cell, where can the DNA be found?

  chromosomes and mitochondria
• DNA MOLECULE IS REPLICATED IN SEMI-CONSERVATIVE MECHANISM

  REPLICATION
• mRNA IS PRODUCED BY COPY- IN THE MESSAGE ‘CODONS’ FROM THE DNA

  TRANSCRIPTION
• SEMI-CONSERVATIVE MECHANISM USING OLD SINGLE STRAND OF DNA TO SYNTHESIZE THE NEW DNA STRAND

  DNA REPLICATION
• INFORMATION (CODONS) FROM THE DNA ARE USED TO SYNTHESIZE THE mRNA AS COMPLEMENTARY SEQUENCE OF THE ANTISENSE DNA STRAND

  TRANSCRIPTION
• THE CODONS IN THE Mrna ARE READ BY tRNA CARRYING THE CORRESPONDING AMINO ACID. THE PROCESS CONTINUES TO MAKE A POLYPEPTIDE UNTIL A STOP CODON IS ENCOUNTERED IN THE mRNA THEN TRANSLATION STOPS.

  TRANSLATION
• ROLE OF SIGNAL PEPTIDE: ALTHOUGH PROTEIN SYNTHESIS STARTS WITH ____ (f-met), THE MATURE PROTEIN IS SHORTENED BY CLEAVING THE SIGNAL PEPTIDE BY A SIGNAL PEPTIDASE BEFORE LOCATING THE PROTEIN IN THE PARTICULAR CELL.

  AUG
• in making a genomic DNA library, ____ is used to check for purity of the DNA


  agarose gel electrophoresis
• Mix restricted genomic DNA with restricted plasmid DNA and add _____ to connect both DNA to make new plasmid constructs.


  DNA ligase
• in making a genomic DNA library, checking for expression of protein is done through the use of

  Mab/Pab
• what stain is used in the extraction of bacterial genomic DNA?

  ethidium bromide
• draw pUC18


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• draw pUC 19


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