ACS Overview

• Chymotrypsin is classified as a protease.
• Chymotrypsin illustrates the principle of transition state stabilization.
• Chymotrypsin is an example of a covalent catalysis and acid-bases catalysis.
• Henderson-Hasselbach Equation

  pH = pKa + log ([A-] / [HA])
• As [H+] increases, Histidine group in hemoglobin becomes more protonated and protein shifts to T-state. O2 binding affinity decreases.
• Mutarotation is the conversion from a to b forms of the sugar at the anomeric carbon.
• Cis fats increase membrane fluidity, while trans fats decrease membrane fluidity.
• Type 1 Integral membrane protein with C-terminus inside and N-terminus outside.
• Type II integral membrane protein with N-terminus inside and C-terminus outside.
• Type III integral membrane protein contains connected protein helices.
• Type IV integral membrane protein with unconnected protein helices.
• A-Form DNA is the condensed form of DNA
• Z-Form DNA is the left-handed helical form of DNA.
• Restriction enzyme cuts DNA at specific restriction sites.
• Enzymes that use TPP as a cofactor: pyruvate decarboxylase, pyruvate dehydrogenase, a-ketoglutarate dehydrogenase, and transketolase.