This video is for paper one of GCSE biology and combined science
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Microscopy
The study of small objects using a microscope
Types of microscopes
Light microscope
Electron microscope
Light microscope
First developed in the mid 17th century
Uses light to form an image
Can be used to view live specimens
Relatively cheap and easy to use
Can magnify up to 2,000 times
Resolution
The ability to see two things as separate objects
Light microscope
Has a low resolution with a resolving power of around 200 nanometers
Electron microscope
Developed by scientists in the 1930s
Uses electrons to form an image
Specimens must be dead
Very expensive and has many conditions
Can magnify up to 2 million times
Has a high resolution of 0.2 nanometers
The electron microscope is much better than the light microscope in terms of magnification and resolution
The high magnification and resolution of the electron microscope allows scientists to see and understand more about the subcellular structures of cells
Animal cell
Contains a nucleus, cell membrane, cytoplasm, mitochondria, and ribosomes
Plant cell
Contains a nucleus, cell membrane, cytoplasm, mitochondria, ribosomes, chloroplasts, permanent vacuole, and cell wall
Animal and plant cells are both eukaryotic cells
Eukaryotic cells
Have a cell membrane, cytoplasm, and genetic material inside a nucleus
Prokaryotic cells
Have a cell membrane and cytoplasm, but their genetic material is not enclosed in a nucleus
Features of prokaryotic cells
Cell membrane
Cytoplasm
Ribosomes
Cell wall (not made of cellulose)
Single loop of DNA
Plasmids
Slime layer
Flagella
Prokaryotic cells do not have chloroplasts or mitochondria
Specialized cells
Cells that have developed different subcellular structures to carry out a particular function
Sperm cell
Has a tail to help it swim
Has many mitochondria in the mid-piece to provide energy
Has an acrosome containing enzymes to break down the egg
Has a large nucleus to contain DNA
Muscle cell
Has many mitochondria to release energy for contraction
Has special proteins that cause contraction by sliding across each other
Can store glycogen to be broken down for respiration
Nerve cell
Has a long axon to carry electrical impulses
Has dendrites to connect to other nerve cells
Has nerve endings that release chemical messengers
Root hair cell
Has a large surface area for absorption
Has a large permanent vacuole to speed up osmosis
Has many mitochondria for active transport of mineral ions
Xylem cell
Forms long hollow tubes to allow easy movement of water and minerals
Has spirals of lignin to strengthen the cell walls and support the plant
Phloem cell
Has sieve plates with holes to allow easy movement of dissolved food
Has adjacent companion cells with many mitochondria to provide energy for food transport
Diffusion
The spreading out of particles in a solution or gas from an area of higher concentration to an area of lower concentration
Diffusion is a passive process that does not require additional energy
Factors affecting rate of diffusion
Temperature
Concentration gradient
Surface area
Osmosis
The diffusion of water molecules from a dilute solution to a more concentrated solution through a partially permeable membrane
Osmosis is a passive process that occurs down a concentration gradient
Osmosis is important in animal cells to maintain the right internal environment
Left side of the membrane
Has more water molecules
Right side of the membrane
Has less water molecules and more sucrose or solute molecules, therefore it is the concentrated side
Osmosis
1. Occurs down a concentration gradient or from a dilute solution to a more concentrated solution
2. Net movement of water is from the left side to the right side
3. Eventually the water concentration will be equal on both sides
4. Water molecules will continue to move back and forth across the partially permeable membrane but there's no further net movement of water, the water is balanced on both sides
Importance of osmosis in animal cells
Ensures solutes like glucose and salts are at the right concentration inside the cell
The internal environment needs to be kept just right for the cell to work
The difference in concentration between the cell's internal environment and the external solution will determine how much osmosis occurs
Effect of different solutions on a red blood cell
1. Hypotonic solution: Water moves into the cell, it stretches and may burst
2. Isotonic solution: No net movement of water, no osmosis occurs
3. Hypertonic solution: Water moves out of the cell, it shrinks and won't function properly
Osmosis is a required practical that looks at the effect of concentration of salt or sugar on the mass of plant tissue
Active transport
Moves substances from a more dilute solution to a more concentrated solution, against the concentration gradient