WEEK 7 : Enzyme Activity of Peroxidase Lab Quiz Prep

• An enzyme is a macro-molecule that has catalytic activity
• A substrate binds at its active site
• Enzymes reduce the activation energy needed to reach the transition state
• Factors that influence enzymatic activity are : pH, ionic strength, temperature, chemical environment
• The kind of chemical bond interactions between substrate and enzyme are due to their weak bonds( ionic, hydrogen bonding)
• Guaiacol turns brown when reacting with hydrogen peroxide and peroxidase because guaiacol is naturally occurring organic compounds made by plants. Its reduced form is colorless but it can be oxidized to turn brown
• It's important for cells to break down hydrogen peroxide because hydrogen peroxide is a toxic compound.