WEEK 9 : Gene Expression Lab Quiz Prep

• The central dogma is the flow of genetic information from DNA to RNA to Protein
• DNA is transcribed into RNA by RNA polymerase, while RNA is translate into protein by ribosomes
• The differences between RNA and DNA are : RNA contains ribose sugar, uracil instead of thymine, and is single stranded. DNA contains deoxyribose sugar, thymine and double helix form
• What strand does RNA Polymerase transcribe?

  Template strand
• Identify and differentiate between 4 levels of protein structure?

  1. Primary : Amino Acid Sequence

  2. Secondary : Alpha helices and beta sheets

  3. Tertiary : Overal 3D structure

  4. Quaternary : Interactions between multiple subunits
• The RNA molecule that carries amino acids to ribosomes are Transfer RNA (tRNA) in which carries amino acids to ribosomes during translation
• What RNA molecule gets transcribed and then read by robosomes?

  Messenger RNA (mRNA) is transcribed from DNA and read by ribosomes during protein synthesis
• The green fluorescence from our lab indicated : Transcription
• The red fluorescence from our lab indicated Translation
• kanamycin interferes with translation by binding to the bacterial ribosome and inhibiting protein synthesis