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practicals
paper 1
enzymes
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Created by
Emily
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Cards (2)
what
is the aim of the experiment?
to determine the
optimum
temperature /
ph
of an enzyme
what is the method?
measure out the
enzyme
and
substrate
( starch and amylase )
place separately in a
water
bath with
buffer
solution
prepare a spotting tile with
iodine
drops
mix reactants and start stopwatch
every 10 seconds remove a drop of the mixture with a
pipette
and put in the spotting tile
when the colour no longer changes the
starch
has been
broken
down
repeat with different buffer solution and plot the times