enzymes

• what is the aim of the experiment?


  to determine the optimum temperature / ph of an enzyme
• what is the method?

  1. measure out the enzyme and substrate ( starch and amylase )
  2. place separately in a water bath with buffer solution
  3. prepare a spotting tile with iodine drops
  4. mix reactants and start stopwatch
  5. every 10 seconds remove a drop of the mixture with a pipette and put in the spotting tile
  6. when the colour no longer changes the starch has been broken down
  7. repeat with different buffer solution and plot the times