enzymes

Cards (2)

  • what is the aim of the experiment?

    to determine the optimum temperature / ph of an enzyme
  • what is the method?
    1. measure out the enzyme and substrate ( starch and amylase )
    2. place separately in a water bath with buffer solution
    3. prepare a spotting tile with iodine drops
    4. mix reactants and start stopwatch
    5. every 10 seconds remove a drop of the mixture with a pipette and put in the spotting tile
    6. when the colour no longer changes the starch has been broken down
    7. repeat with different buffer solution and plot the times