LIVER

• main function of liver 1 : Hepatic Synthesis Ability
• main function of liver 2 : excretion function
• main function of liver 3: Detoxification Function
• Malloy-Evelyn Method reagent : 50% methanol
• Jendrassik-Grof Method reagent : Caffeine-benzoate-acetate
• Bilirubinometry is used in neonatal population measuring of reflected light from skin using two wavelengths
• Icterus Index - Yellowishness of the serum or plasma
• conjugated bilirubin : 0.0-0.2 mg/dl
• unconjugated bilirubin: 0.2-0.8 mg/dl
• total bilirubin : 0.2-1.0 mg/dl
• Serum is preferred for Malloy-Evelyn
• Addition of alcohol may precipitate protein – interference!
• Fasting sample is preferred - Presence of lipemia may interfere
• icterus index reagent : 0.01% potassium dichromate
• advantage of Jendrassik-Grof over Malloy-Evelyn: Is not affected by hemoglobin up to 750 mg/dL
• advantage of Jendrassik-Grof over Malloy-Evelyn: Insensitive to a 50-fold variation in protein concentration of the sample
• Delta Bilirubin - conjugated bilirubin binded to albumin and seen only in hepatic obstruction
• Classic Diazo reaction: Urine bilirubin reacts to diazotized sulfanilic acid to produce red azo dye (540 nm)
• alkaline tartrate solution unconjugated = Total bilirubin-conjugated
• Van den Bergh - Used alcohol accelerator for the coupling of bilirubin to diazotized sulfanilic acid
• Bilirubin Assay 1: Malloy-Evelyn Method
• Bilirubin Assay 2: Jendrassik-Grof Method
• Urobilinogen : Ehrlich Method
• Bromosulfophthalein Test (BSP)3: MacDonald Method
• Bromosulfophthalein Test (BSP)2: Rosenthal White Method
• Bromosulfophthalein Test (BSP) 1 -Dye Extraction Test
• extraction function 1: Bilirubin Assay
• extraction function 2: Bromosulfophthalein Test
• extraction function 3: Urobilinogen
• Detoxification Function 1: ammonia
• Detoxification Function 2: Berthelot Reaction
• Nesslerization Reaction produces brown compound