MABs

• Monoclonal antibody


  Antibodies produced from a single clone of hybridoma cells, specific to one binding site on one protein antigen
• Production of monoclonal antibodies


  1. Inject mouse with antigen

  2. Collect lymphocytes from mouse

  3. Fuse lymphocyte with tumor cell to form hybridoma

  4. Select single hybridoma cell producing desired antibody

  5. Allow hybridoma cell to divide by mitosis to form clone
• Monoclonal antibodies


  • Specific to one binding site on one protein antigen
  • Can target specific chemicals or cells in the body
  • Have a large number of uses
• Lymphocytes produce antibodies against foreign objects called antigens
• Lymphocytes will not divide by mitosis on their own
• Hybridoma cells can produce antibodies and divide by mitosis
• Monoclonal antibodies are produced from a clone of identical hybridoma cells
• A large amount of monoclonal antibody can be collected and purified