SPECIAL STAINS

• Stains for carbohydrates

  • Periodic Acid Shiff/PAS
  • Best Carmine
  • Langhan's lodine Method (Carleton's Method)
  • Fresh Frozen azure A metachromic stain
  • Metachromatic Toluidine Blue staining
  • Alcian Blue Technique
  • Combined alcian blue - PAS Technique
  • Hale's Cialyzed (colloidal) Iron technique
  • Mucicarmine Stain
  • Fluorescent Acridine Orange Technique
  • Southgate's Mucicarmine Technique
• Stains for proteins

  • Alkaline Fast Green Method
  • Peracetic Acid - Alcian Blue
  • Sakaguchi's Test
• Stains for fats or lipids

  • Sudan Black
  • Sudan IV (Scharlach R)
  • Oil Red O Method Dextrin
  • Osmic Acid Stain
  • Nile Blue Sulfate
  • Toluidine Blue Acetone Method
  • Borohydride Periodic Schiff (BHPS)
• Stains for enzymes

  • Gomori Calcium Method
  • Gomori Lead Method
  • Lead Method for 5-nucleotidase (Wachstein & Meisel)
  • Alpha naphthyl acetate Method for non-specific esterases
  • Indoxyl acetate Method for non-specific esterase (Holt & Withers)
  • Tetrazolium method for monoamine oxidase
• Stains for bone marrow & blood elements

  • Rapid Toluidine - Eosin Stain for glycol methacrylate section
  • Wright's - Giemsa - Jenner stain
  • Peroxidase Reaction for Myeloid cells
• Stains for connective tissue

  • Gomori's Silver Impregnation
  • Van Gieson
  • Masson's Trichrome Stain
  • Weigert's Elastic Tissue Stain
  • Orcein (Taenzer-Unna-Orcein Method)
  • Kraiani's Technique (employing Congo Red)
  • Mallory's Phosphotungstic Acid Hematoxin (PTAH) Method
  • Highman's Congo Red
  • Akaline Congo Red Technique
  • Kraljan's Amyloid Stain (modified Bennhold Method)
  • Methyl Violet - Crystal Violet Method
  • Induced Fluorescent Staining with Thioflavine T
• Stains for nucleic acids

  • Feulgen's technique for Nuclear DNA
  • Methyl Green-Pyronin method
  • Acridine Orange Fluorescent Staining
• Stains for muscle and bone

  • Modified Gomori's Trichrome
  • Mallory's Phosphotungstic Acid Hematoxylin (PTAH)
  • Heidenhain's Iron Hematoxylin
  • Lissamine Fast Red - Tartrazine Method
  • Schnorls's Picro Thionin Method
• Stains for central nervous system

  • Bielschowsky Technique
  • Bodian's Stain
  • Sevier Munger Technique
  • Cresyl Fast Violet (Nissl) Stain for paraffin sections
  • Weigert Pal Technique of Staining Normal Myelin
  • Kluver & Barrera Luxol Fast Blue Stain for myelin with Nissl Counterstain
  • Luxol Fast Blue - H & E Stain
  • Luxol Fast Blue - PAS Hematoxylin Stain
  • Weil's Method
  • Cajal's Gold Sublimate
  • Modified Holzer's Method for Astrocytic Processes
• Stains for tissue pigments and deposits

  • Perl's Prussian Blue
  • Gomori's Prussian Blue
  • Tumbull's Blue Reaction for ferrous iron (hemosiderin)
  • Benzidine Nitroprusside Stain
  • Modified Fouchet's Technique
  • Schmorl's Ferric Ferricyanide method for reducing substances
  • Gomori's Aldehyde Fuchsin
  • Mallory's Fuchsin stain
  • Masson Fontana Technique
  • Calcium Dye Lake Reaction
  • Von Kossa's Silver Nitrate Method
  • Lindquist's Modified Rhodamine Technique
• Additional stains


  • Alizarin Red S
  • Rubeanic acid
  • Modified Rhodanine
  • Rhodizonate method
  • Sirius Red
  • Gimenez method
  • Toluidine blue in Sorenson's buffer
  • Modified Steiner
  • Macchiavello's stain
  • Shikata's orcein method
  • Goldner's method
  • Modified Palmgren's
  • Page's solochrome cyanine technique
  • Perchloric acid-naphthoquinone
  • Sodium hydroxide-ferric hematoxylin
  • Toluidine blue-acetone
  • Weigert's resorcin-fuchsin
  • Gordon's and Sweet's